Metabolic stability or clearance is recognized as one of the main determinants of drug concentration in blood and has been used effectively to predict pharmacokinetics and toxicokinetics.
Compounds with poor metabolic stability are exposed to comprehensive first-pass metabolism in liver and gut right after absorption from the gut via portal vein, causing poor overall bioavailability to circulation and rapid elimination from the body.
In vitro studies on metabolic stability are conducted to measure the rate of disappearance of the molecule using liver microsomes, S9 fraction or isolated hepatocytes. The in vitro data are very valuable to predict the in vivo hepatic clearance and estimating bioavailability and systemic clearance
Quintara Discovery offers a variety of metabolic stability assays to determine the in vitro half-life and intrinsic clearance of test compounds. The assays can be conducted using different matrices, including liver microsomes, S9, hepatocytes, plasma, and blood, all from various species.
Quintara Discovery also offers metabolic stability assays in various buffers, such as simulated intestinal and gastric fluids.