Quintara Discovery

Hepatocyte Stability

Hepatocytes are a living system, which closely mimics the in vivo situation. They contain all the liver metabolizing enzymes and their cofactors. It is a preferred system for in vitro drug metabolism studies.

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Hepatocyte Stability Assays: Standard Assay Conditions (Customizable)

Compound Concentration 1 µM
Hepatocyte density 500,000 cells/mL
Time Points Time points (0, 60, 120, 180 minutes) for half-life and intrinsic clearance determinations; zero and 60 minutes for %disappearance of parent compound
Detection Method LC-MS/MS
Control compounds Midazolam
Species Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables %disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time 2-3 Days
Compound requirements 20 μL 10 mM DMSO stock