Hepatocyte Stability

Hepatocytes are a living system, which closely mimics the in vivo situation. They contain all the liver metabolizing enzymes and their cofactors. It is a preferred system for in vitro drug metabolism studies.

Hepatocyte Stability Assays: Standard Assay Conditions (Customizable)

Compound Concentration	1 µM
Hepatocyte density	500,000 cells/mL
Time Points	Time points (0, 60, 120, 180 minutes) for half-life and intrinsic clearance determinations; zero and 60 minutes for %disappearance of parent compound
Detection Method	LC-MS/MS
Control compounds	Midazolam
Species	Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables	%disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time	2-3 Days
Compound requirements	20 μL 10 mM DMSO stock