Quintara Discovery

S9 Stability

S9 contains all the enzymes present in the microsomes and cytosol, such as aldehyde oxidase and many enzymes responsible for phase II metabolism.

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S9 Stability Assays: Standard Assay Conditions (Customizable)

Compound Concentration 1 µM
S9 Concentration 2 mg protein/mL
Time Points Time points (0, 15, 30, 60 minutes) for half-life and intrinsic clearance determinations; zero and 30 minutes for %disappearance of parent compound
Detection Method LC-MS/MS
Control compounds Verapamil
Species Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables %disappearance of parent compound, Half-life (t1/2) and intrinsic clearance (CLint)
Turnaround Time 2-3 Days
Compound requirements 20 μL 10 mM DMSO stock