Quintara Discovery’s metabolite profiling and identification approach always starts with a well-designed compound incubation. Choosing the relevant in vitro systems, such as liver microsomes, S9, or hepatocytes, as well as in vivo systems, and executing with precision and rigor are prerequisites for a successful MetID effort. Metabolites generated are investigated by LC/MS/MS on Q-TRAP and LC/UV. Detailed analysis will aim to identify the type of metabolism, the soft spot, and the chemical reaction that lead to the specific metabolite formation. Chemical structural elucidation of the metabolite can be achieved.