Tissue Protein Binding

The binding of drug to tissue (such as rat brain) proteins is a major determinant of drug disposition and plays an important effect on pharmacodynamics since only the free (unbound) drug is responsible for the pharmacological activity.

Assay Methodology

At Quintara Discovery, tissue protein binding for a test compound is determined using the Rapid Equilibrium Dialysis (RED) method performed in a 48-well format.

Tissue Protein Binding: Standard Assay Conditions (Customizable)

Testing Compound Concentration	1 μM
Dialysis buffer	PBS, pH 7.4
Incubation Period	4 hour at 37 °C, 10% CO2 if needed
Detection Method	LC-MS/MS
Control Compound	Verapamil
Species	Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables	% Bound
Turnaround Time	2-3 Days
Compound requirements	20 μL 10 mM DMSO stock