The binding of drug to tissue (such as rat brain) proteins is a major determinant of drug disposition and plays an important effect on pharmacodynamics since only the free (unbound) drug is responsible for the pharmacological activity.
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Assay Methodology
At Quintara Discovery, tissue protein binding for a test compound is determined using the Rapid Equilibrium Dialysis (RED) method performed in a 48-well format.
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Tissue Protein Binding: Standard Assay Conditions (Customizable)
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Testing Compound Concentration |
1 μM |
Dialysis buffer |
PBS, pH 7.4 |
Incubation Period |
4 hour at 37 °C, 10% CO2 if needed |
Detection Method |
LC-MS/MS |
Control Compound |
Verapamil |
Species |
Human, rat, mouse, dog and monkey (additional species are available on request) |
Deliverables |
% Bound |
Turnaround Time |
2-3 Days |
Compound requirements |
20 μL 10 mM DMSO stock |
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