Quintara Discovery

Tissue Protein Binding

The binding of drug to tissue (such as rat brain) proteins is a major determinant of drug disposition and plays an important effect on pharmacodynamics since only the free (unbound) drug is responsible for the pharmacological activity.

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Assay Methodology

At Quintara Discovery, tissue protein binding for a test compound is determined using the Rapid Equilibrium Dialysis (RED) method performed in a 48-well format.

Tissue Protein Binding: Standard Assay Conditions (Customizable)

Testing Compound Concentration 1 μM
Dialysis buffer PBS, pH 7.4
Incubation Period 4 hour at 37 °C, 10% CO2 if needed
Detection Method LC-MS/MS
Control Compound Verapamil
Species Human, rat, mouse, dog and monkey (additional species are available on request)
Deliverables % Bound
Turnaround Time 2-3 Days
Compound requirements 20 μL 10 mM DMSO stock